Rabbit polyclonal antibody to B-RAF (Phospho-Ser446)
Specificity
The antibody detects endogenous level of B-RAF only when phosphorylated at Serine 446.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 446 (R-D-S(p)-S-D) derived from Human B-RAF.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from 293 cells treated with EGF using B-RAF (Phospho-Ser446) Antibody #12064.The lane on the left is treated with the antigen-specific peptide.
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