Anti-Biotin Antibody [BTN399] - BSA and Azide free (A278454)

Anti-Biotin Antibody [BTN399] - BSA and Azide free

Mouse monoclonal [BTN399] antibody to Biotin.

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 0.5-1 µg/million cells, IF: 0.5-1 µg/ml, WB: 0.5-2 µg/ml, IHC-P: 0.5-1 µg/ml

Biotinylated sheep immunoglobulin.

Mouse

Monoclonal

BTN399

IgG1

kappa

Protein A/G chromatography.

1 mg/ml

244Da

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Biotin Antibody [BTN399] (A277866).