ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with Biotinylated Human IgG (0.01 µg/ml) and blocked. Then either Anti-Biotin Antibody [1D4-C5] (A320690) (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or Streptavidin-HRP (C) was added. HRP signal increased in dose dependent manner, but with different dynamics, when biotinylated antibody was detected by Anti-Biotin Antibody [1D4-C5] (A320690)/goat anti mouse-HRP (A) or by Streptavidin-HRP (B), and it was falling to zero in dose dependent manner when Streptavidin-HRP was competed by Anti-Biotin Antibody [1D4-C5] (A320690), (C).
Publishing research using Anti-Biotin Antibody [1D4-C5] (A320690)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-Biotin Antibody [1D4-C5] (A320690)
![ELISA - Anti-Biotin Antibody [1D4-C5] (A320690)](https://cdn.antibodies.com/image/catalog/320/A320690_1.jpg?profile=product_top)
![ELISA - Anti-Biotin Antibody [1D4-C5] (A320690)](https://cdn.antibodies.com/image/catalog/320/A320690_1.jpg?profile=product_top_thumb)
![ELISA - Anti-Biotin Antibody [1D4-C5] (A320690)](https://cdn.antibodies.com/image/catalog/320/A320690_1.jpg?profile=product_image)
![Immunohistochemistry - Anti-Biotin Antibody [Hyb-8] (A250875) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250876_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Biotin Antibody [Hyb-8] - BSA and Azide free (A254055) - Antibodies.com](https://cdn.antibodies.com/image/catalog/254/A254056_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Biotin Antibody [SPM375] (A250903) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250904_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Biotin Antibody [BTN399] - BSA and Azide free (A278454) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278454_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Biotin Antibody [BTN399] (A277866) - Antibodies.com](https://cdn.antibodies.com/image/catalog/277/A277866_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Biotin Antibody [SPM375] - BSA and Azide free (A254083) - Antibodies.com](https://cdn.antibodies.com/image/catalog/254/A254084_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Biotin Antibody [BTN/36] (A277869) - Antibodies.com](https://cdn.antibodies.com/image/catalog/277/A277869_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Biotin Antibody [BTN/403] (A277867) - Antibodies.com](https://cdn.antibodies.com/image/catalog/277/A277867_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Biotin Antibody [BTN/36] - BSA and Azide free (A278457) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278457_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Biotin Antibody [BTN/403] - BSA and Azide free (A278455) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278455_1.jpg?profile=product_alternative)
