BRAF expression in lysates from wild type (WT), 293T cells transfected with BRAF and 293T cells transfected with BRAF V600E analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-BRAF Antibody (A329154) at a dilution of 1:400 and detected with chemiluminescence.
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