Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, IHC-P: 1-2 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM Tris buffer containing 1 mM EDTA, pH 9.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes.