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Anti-c-Myc (phospho Ser62) Antibody [33A12E10] (A407)

Identification of cMyc protein whose Ser62 is phosphorylated by Western blotting Immunofluorescence staining of cMyc phosho-Ser62 in nuclei of HeLa cells. 1. HeLa cells were fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block non-specific binding of the antibodies. Incubate the cells with 1/2,000 diluted anti-cMyc p62 antibody in 1% BSA in PBS at 4? overnight. 3. Incubate cells with a secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPI Immunofluorescence staining of cMyc phosho-Ser62 in HeLa cells
$555
Product Datasheet

Mouse monoclonal (33A12E10) antibody to c-Myc (phospho Ser62) for WB, IF, IHC-P, FC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-c-Myc (phospho Ser62) Antibody [33A12E10]
Description
Mouse monoclonal (33A12E10) antibody to c-Myc (phospho Ser62).
Applications
WB, IF, IHC-P, FC, ELISA
Dilutions
WB: 1 µg/ml, IF: 0.5-1 µg/ml, IHC: 5 µg/ml
Reactivity
Human, Mouse
Immunogen
Synthetic peptide containing phospho Ser62 of c-Myc.
Host
Mouse
Clonality
Monoclonal
Clone ID
33A12E10
Isotype
IgG2b
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol (without Sodium Azide and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BHLHE39, Class E basic helix-loop-helix protein 39, MYC, Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Identification of cMyc protein whose Ser62 is phosphorylated by Western blottingEnlarge Image

Anti-c-Myc (phospho Ser62) Antibody [33A12E10] (A407)

Anti-c-Myc (phospho Ser62) Antibody
Immunofluorescence staining of cMyc phosho-Ser62 in nuclei of HeLa cells. 1. HeLa cells were fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block non-specific binding of the antibodies. Incubate the cells with 1/2,000 diluted anti-cMyc p62 antibody in 1% BSA in PBS at 4? overnight. 3. Incubate cells with a secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPIEnlarge Image

Immunofluorescence - Anti-c-Myc (phospho Ser62) Antibody [33A12E10] (A407)

Anti-c-Myc (phospho Ser62) Antibody
Immunofluorescence staining of cMyc phosho-Ser62 in HeLa cellsEnlarge Image

Immunofluorescence - Anti-c-Myc (phospho Ser62) Antibody [33A12E10] (A407)

Anti-c-Myc (phospho Ser62) Antibody
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Proteins predicted to interact with c-Myc

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.8% Relevancy Score
99.7% Relevancy Score