The antibody recognizes Ig domain D2 (membrane proximal) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes.
Applications
Flow Cytometry, WB, Functional Studies
Dilutions
FUNC: This antibody (high-affinity mAb of unique epitope specificity) inhibits anti-CD3-induced T cell activation, Flow Cytometry: 1-4 µg/ml, WB: Positive Control: 293 human fibroblastoid cell line, Non-reducing conditions.
Reactivity
Human
Immunogen
Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimmer).
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-M6/6
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
50-60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide (0.2 µm filter sterilized). Endotoxin level is less than 0.01 EU/µg of the protein.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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