Anti-CD16 Antibody [DJ130c] (A280258)

Anti-CD16 Antibody [DJ130c]

Mouse monoclonal [DJ130c] antibody to CD16.

This antibody recognises human CD16, also known as Low affinity immunoglobulin gamma Fc region receptor III-A or Fc-gamma RIIIa. CD16a is a 254 amino acid ~50-65 kDa single pass type 1 transmembrane glycoprotein bearing two Ig-like C2 type domains. CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form, Fc gammaRIIIB, or CD16B. CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes. In addition, CD16B exists as two allelic variants NA1 and NA2. DJ130c recognises all polymorphonuclear cells irrespective of their NA phenotype.recognises an epitope in the first membrane-distal domain of CD16, recognises both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta

IHC-P, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:20 for 25 µg, 1:50 for 0.2 mg, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Human, Macaque

Mouse

Monoclonal

DJ130c

IgG1

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD16 antibody, clone DJ130c recognizes human CD16, also known as Low affinity immunoglobulin gamma Fc region receptor III-A or Fc-gamma RIIIa. CD16a is a 254 amino acid ~50-65 kDa single pass type 1 transmembrane glycoprotein bearing two Ig-like C2 type domains. CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form, Fc gammaRIIIB, or CD16B (Scallon et al. 1989). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes (Ravetch and Perussia 1989). In addition, CD16B exists as two allelic variants NA1 and NA2 . DJ130c recognizes all polymorphonuclear cells irrespective of their NA phenotype.Mouse anti Human CD16 antibody, clone DJ130c recognizes an epitope in the first membrane-distal domain of CD16, recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta (Xu et al. 2012)

CD16-II, CD16a, CD16a antigen, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FCG3, FcgammaRIIIA, FCGR3, FCGR3A, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, IgG Fc receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-A