This antibody recognizes CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.
Applications
Flow Cytometry, IP, IHC-P, IHC-Fr
Dilutions
Flow Cytometry: 1-4 µg/ml
Reactivity
Human
Immunogen
A cell membrane antigen preparation that was isolated from normal human thymocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
SN13
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
44 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using Anti-CD1b Antibody [SN13] (A85923), (concentration in sample 9 µg/ml, GAM PE) and Anti-CD14 Antibody [MEM-15] (Pacific Blue), (4 µl reagent per milion cells in 100 µl of cell suspension).
Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using Anti-CD1b Antibody [SN13] (A85923), (concentration in sample 9 µg/ml, GAM PE) and Anti-HLA DR Antibody [L243] (APC) (A86732), (10 µl reagent per milion cells in 100 µl of cell suspension).