This antibody recognizes CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
A cell membrane antigen preparation that was isolated from normal human thymocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
SN13
Isotype
IgG1
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
44 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells labeling CD1b with Anti-CD1b Antibody [SN13] (APC) (A320698), (10 µl reagent per milion cells in 100 µl of cell suspension).
Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using Anti-CD1b Antibody [SN13] (APC) (A320698), (10 µl reagent per milion cells in 100 µl of cell suspension) and Anti-CD11c Antibody [BU15] (PE) (A86672), (20 µl reagent per milion cells in 100 µl of cell suspension).
Separation of human CD11c positive CD1b positive cells (red-filled) from CD11c negative CD1b negative cells (black-dashed) in flow cytometry surface staining of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells, labeling CD1b with Anti-CD1b Antibody [SN13] (APC) (A320698), (10 µl reagent per milion cells in 100 µl of cell suspension).
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Proteins predicted to interact with CD1b
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
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