This antibody recognizes CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
A cell membrane antigen preparation that was isolated from normal human thymocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
SN13
Isotype
IgG1
Light Chains
kappa
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
44 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human CD1b positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4), (red) from CD1b negative monocytes (blue) in flow cytometry surface staining of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells, labeling CD1b with Anti-CD1b Antibody [SN13] (FITC) (A85922), (4 µl reagent per milion cells in 100 µl of cell suspension).
Publishing research using Anti-CD1b Antibody [SN13] (FITC) (A85922)? Please let us know so that we can list the citation on this page.
Proteins predicted to interact with CD1b
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-CD1b Antibody [SN13] (FITC) (A85922)](https://cdn.antibodies.com/image/catalog/85/A85922_1.jpg?profile=product_top)
![Flow Cytometry - Anti-CD1b Antibody [SN13] (FITC) (A85922)](https://cdn.antibodies.com/image/catalog/85/A85922_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD1b Antibody [SN13] (FITC) (A85922)](https://cdn.antibodies.com/image/catalog/85/A85922_1.jpg?profile=product_image)