Anti-CD3 epsilon Antibody [APA1/1] (A85568)

Anti-CD3 epsilon Antibody [APA1/1]

Mouse monoclonal [APA1/1] antibody to CD3 epsilon.

This antibody recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Flow Cytometry, IP, WB, IHC-Fr, ICC

Flow Cytometry: Intracellular staining: 1-4 µg/ml; Positive Control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 µg/ml). Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0, 1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 µg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS, IHC: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.

Mouse, Human

Purified human CD3 proteins isolated from thymus.

Mouse

Monoclonal

APA1/1

Protein A chromatography.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E