CD31 expression in Jurkat cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-CD31 Antibody (A326230) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cell junction staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
CD31 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-CD31 Antibody (A326230) at 4µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for CD31 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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