This antibody reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells. It is gradually lost on progenitor cells. This antibody completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Western bloting analysis of human CD34 using Anti-CD34 Antibody [4H11[APG]] (A85518) on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD34 monoclonal antibody 4H11[APG] followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD34 protein at approximately 110 kDa.
Publishing research using Anti-CD34 Antibody [4H11[APG]] (A85518)? Please let us know so that we can list the citation on this page.
![Flow Cytometry - Anti-CD34 Antibody [4H11[APG]] (A85518)](https://cdn.antibodies.com/image/catalog/85/A85518_1.jpg?profile=product_top)
![WB - Anti-CD34 Antibody [4H11[APG]] (A85518)](https://cdn.antibodies.com/image/catalog/85/A85518_2.jpg?profile=product_top)
![Flow Cytometry - Anti-CD34 Antibody [4H11[APG]] (A85518)](https://cdn.antibodies.com/image/catalog/85/A85518_1.jpg?profile=product_top_thumb)
![WB - Anti-CD34 Antibody [4H11[APG]] (A85518)](https://cdn.antibodies.com/image/catalog/85/A85518_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD34 Antibody [4H11[APG]] (A85518)](https://cdn.antibodies.com/image/catalog/85/A85518_1.jpg?profile=product_image)
![WB - Anti-CD34 Antibody [4H11[APG]] (A85518)](https://cdn.antibodies.com/image/catalog/85/A85518_2.jpg?profile=product_image)
![Flow Cytometry - Anti-CD34 Antibody [QBEnd-10] - BSA and Azide free (A85659)](https://cdn.antibodies.com/image/catalog/85/A85659_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD34 Antibody [QBEnd/10] (A250677) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250677_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD34 Antibody [HPCA1/1171] - BSA and Azide free (A253861) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253861_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD34 Antibody [QBEND/10] (A281617) - Antibodies.com](https://cdn.antibodies.com/image/catalog/281/A281617_4.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD34 Antibody [HPCA1/2598R] (A250684) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250684_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD34 Antibody [HPCA1/2598R] - BSA and Azide free (A253864) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253864_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD34 Antibody [HPCA1/1171] (A250681) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250681_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-CD34 Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323939) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323939_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD34 Antibody [SPM610] (A250681) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250682_1.jpg?profile=product_alternative)