Anti-CD34 Antibody [QBEnd-10] - BSA and Azide free
Description
Mouse monoclonal [QBEnd-10] antibody to CD34.
Specificity
This antibody reacts with an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
Separation of human CD45dim CD34 positive stem cells (red-filled) from human lymphocytes (black-dashed) in flow cytometry surface staining of peripheral whole blood, labeling CD34 with Anti-CD34 Antibody [QBEnd-10] - BSA and Azide free (A85659), (concentration in sample 0,6 µg/ml, GAM APC).
Anti-CD34 Antibody [QBEnd-10] - BSA and Azide free (A85659)
Western blotting analysis of human CD34 using Anti-CD34 Antibody [QBEnd-10] - BSA and Azide free (A85659) on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD34 monoclonal antibody QBEnd-10 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD34 protein at approximately 110 kDa.
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