The antibody recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.
Applications
Flow Cytometry, IP
Dilutions
IP: Excellent, Flow Cytometry: 3 µg/ml. Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells.
Reactivity
Human
Immunogen
Human thymocytes and T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-115
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunoprecipitation of human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by Anti-CD4 Antibody [MEM-241] (A86170). Lane 1: Original lysate of T cells. Lane 2: immunoprecipitate by negative control antibody. Lane 3: Immunoprecipitate by Anti-CD4 Antibody [MEM-115] (A85638).
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