Anti-CD4 Antibody [MEM-115] - BSA and Azide free (A85641)

Anti-CD4 Antibody [MEM-115] - BSA and Azide free

Mouse monoclonal [MEM-115] antibody to CD4.

The antibody recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.

Flow Cytometry, IP, Functional Studies

FUNC: This antibody blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions, IP: Excellent, Flow Cytometry: 3 µg/ml. Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells.

Human

Human thymocytes and T lymphocytes.

Mouse

Monoclonal

MEM-115

IgG2a

Protein A chromatography.

1 mg/ml

55 kDa

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4