Recombinant chimeric monoclonal [7E1] antibody to CD45.
Applications
IHC
Dilutions
IHC: 1:100
Reactivity
Human
Host
Rabbit / Human
Clonality
Monoclonal
Clone ID
7E1
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
A. Anti-CD45 Antibody [7E1] at 10µg/ml staining of CD45 in Hut78 cells by IHC. B. Anti-CD45 Antibody [7E1] at 10µg/ml staining of CD45 in human tonsil tissue by IHC.
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