Anti-CD45RC Antibody [OX-22] (A281417)

Anti-CD45RC Antibody [OX-22]

Mouse monoclonal [OX-22] antibody to CD45RC.

This antibody recognises rat CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like). This product is routinely tested in flow cytometry on rat splenocytes.

IHC-P, IHC-Fr, Flow Cytometry

Flow Cytometry: 1:100, IHC-Fr: 1:100 - 1:1,000, IHC-P: 1:100 - 1:1,000, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

Phytohaemagglutinin activated rat lymphocytes.

Mouse

Monoclonal

OX-22

IgG1

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Rat CD45RC antibody, clone OX-22 recognizes rat CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like). This product is routinely tested in flow cytometry on rat splenocytes.