Recombinant rabbit monoclonal [DM44] antibody to CD48.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM44
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
B-lymphocyte activation marker BLAST-1, BCM1, BCM1 surface antigen, BLAST1, CD48 antigen, Leukocyte antigen MEM-102, Signaling lymphocytic activation molecule 2, SLAM family member 2, SLAMF2, TCT.1
Expi 293 cell line was transfected with irrelevant protein (A) and human CD48 (B) were surface stained with Anti-CD48 Antibody [DM44] - BSA and Azide free (A318651) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Flow cytometry data of serially titrated Anti-CD48 Antibody [DM44] - BSA and Azide free (A318651) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Flow cytometry analysis of antigen binding of Anti-CD48 Antibody [DM44] - BSA and Azide free (A318651). (A) Anti-CD48 Antibody [DM44] - BSA and Azide free (A318651) does not bind to 293T cells that do not express CD48. (B) A clear peak shift of Anti-CD48 Antibody [DM44] - BSA and Azide free (A318651) was seen compared to the control when incubated with CD48-expressing Raji cells, indicating strong binding of Anti-CD48 Antibody [DM44] - BSA and Azide free (A318651) to CD48. Antibodies were incubated at 2 µg/ml. Anti-CD48 antibody (Anti-CD48 Antibody [DM44] - BSA and Azide free (A318651) at 1/1000 dilution.
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