Anti-CD53 Antibody [161-2] (A250738)

Anti-CD53 Antibody [161-2]

Mouse monoclonal [161-2] antibody to CD53.

This antibody recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This antibody recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

Functional Studies, Flow Cytometry, IF

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Human

This antibody does not cross react with Baboon or Horse.

Stimulated human leukocytes.

Mouse

Monoclonal

161-2

IgG2a

Protein A/G chromatography.

200 µg/ml

33-55 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD53 Antibody [161-2] - BSA and Azide free (A253918).

Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin-25, Tspan-25, TSPAN25