Anti-CD53 Antibody [63-5A3] (A250737)

Anti-CD53 Antibody [63-5A3]

Mouse monoclonal [63-5A3] antibody to CD53.

This antibody recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.

Functional Studies, Flow Cytometry, IF

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Human

Human Sezary cells.

Mouse

Monoclonal

63-5A3

IgG2b

Protein A/G chromatography.

200 µg/ml

33-55 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD53 Antibody [63-5A3] - BSA and Azide free (A253917).

Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin-25, Tspan-25, TSPAN25