Recombinant rabbit monoclonal [2A3] antibody to CD7.
Applications
WB
Dilutions
WB: 1:1,000
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
2A3
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Western blot analysis of CD7 in Jurkat, whole cell lysate using Anti-CD7 Antibody [2A3] at 1:1,000 dilution with Goat Anti-Rabbit IgG H&L (HRP) at 1:5,000 dilution. The predicted band weight was 25 kDa, and the observed band weight was 33 kDa.
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