Mouse monoclonal [C8/144B] antibody to CD8 alpha (CF®680).
Applications
IHC, IHC-P
Positive Control
HuT78 cells, human peripheral blood lymphocytes (hPBL), and tonsil tissue.
Dilutions
IF: 0.5-1 µg/mL, IHC-P: 1-2 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Flow cytometry is not recommended.
Reactivity
Human
Immunogen
13-amino-acid peptide derived from the C-terminal cytoplasmic domain of human CD8 alpha.
Host
Mouse
Clonality
Monoclonal
Clone ID
C8/144B
Isotype
IgG1
Light Chains
kappa
Conjugate
CF®680
Concentration
0.1 mg/mL
Predicted MW
32 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
