Western blotting analysis of human Cdk1 using Anti-CDK1 Antibody [POH-1] (A86650) on lysates of HeLa, MOLT-4, and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-Cdk1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for Cdk1 protein at approximately 32 kDa.
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