Western Blot - Anti-CDK1 Antibody [ARC50607] (A305462)
Western blot analysis of extracts of various cell lines, using Anti-CDK1 Antibody [ARC50607] (A305462) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-CDK1 Antibody [ARC50607] (A305462)
Western blot analysis of extracts of Mouse spleen, using Anti-CDK1 Antibody [ARC50607] (A305462) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunofluorescence analysis of HeLa using Anti-CDK1 Antibody [ARC50607] (A305462) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 using Anti-CDK1 Antibody [ARC50607] (A305462) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS using Anti-CDK1 Antibody [ARC50607] (A305462) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-CDK1 Antibody [ARC50607] (A305462)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-CDK1 Antibody [ARC50607] (A305462). This Western blot was performed on the immunoprecipitate using Anti-CDK1 Antibody [ARC50607] (A305462) at a dilution of1:1000.
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