Cetuximab Biosimilar - Anti-EGFR Antibody - BSA and Azide free
Description
Recombinant chimeric monoclonal antibody to EGFR.
Applications
ELISA
Dilutions
ELISA: 1:5,000-10,000
Reactivity
Human
Host
Chimeric
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plate pre-coated by 2 µg/ml (100 µl/well) Recombinant Human EGFR Protein (6xHis Tag) (A318318) can bind Anti-EGFR Chimeric Antibody [Cetuximab Biosimilar] - Azide free (A318922) in a linear range of 0.64-80 µg/ml.
EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR Chimeric Antibody [Cetuximab Biosimilar] - Azide free (A318922) on Expi293 cells transfected with human EGFR (blue histogram) or Expi293 transfected with irrelevant protein (red histogram), and isotype control on Expi293 transfected with irrelevant protein (orange histogram).
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