Immunofluorescence analysis of NIH/3T3 cells using Anti-CTCF Antibody (A13288) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-CTCF Antibody (A13288) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-CTCF Antibody (A13288) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-CTCF Antibody (A13288). This Western blot was performed on the immunoprecipitate using Anti-CTCF Antibody (A13288) at a dilution of 1:500.
Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-CTCF Antibody (A13288) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
