CYP1A1 expression in lysates from wild type (WT) and 293F cells transfected with CYP1A1 analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-CYP1A1 Antibody (A329301) at a dilution of 1:1000 and detected with chemiluminescence.
CYP1A1 expression in mouse kidney tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-CYP1A1 Antibody (A329301) at a dilution of 1:200.
CYP1A1 expression in human colon carcinoma tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-CYP1A1 Antibody (A329301) at a dilution of 1:200.
CYP1A1 expression in Mouse liver tissue analyzed by immunofluorescence. Staining was performed with Anti-CYP1A1 Antibody (A329301) at a dilution of 1:200 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L)secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
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