CYP1A1 expression in Rat Lung lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-CYP1A1 Antibody (A82490) at 0.1µg/ml and detected by chemiluminescence.
CYP1A1 expression in Human Lung lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-CYP1A1 Antibody (A82490) at 0.1µg/ml and detected by chemiluminescence.
Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in Tris/EDTA buffer, pH 9. Staining was performed with Anti-CYP1A1 Antibody (A82490) and revealed with horseradish peroxidase (HRP) at a dilution of 1:500.
Negative control for CYP1A1 expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
CYP1A1 expression in NIH3T3 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-CYP1A1 Antibody (A82490) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
