Anti-Cytokeratin 18 Antibody (A90111)

Name: Anti-Cytokeratin 18 Antibody
Brand: Antibodies.com
SKU: A90111
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Cytokeratin 18 for WB, IHC-P, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Epithelial Cell Marker Hepatocyte Marker

Specifications

Name

Anti-Cytokeratin 18 Antibody

Description

Rabbit polyclonal antibody to Cytokeratin 18.

Applications

WB, IHC-P, ICC/IF, ELISA

Positive Control

HeLa, HepG2.

Dilutions

WB: 1:1,000-1:5,000, IHC-P: 1:50-1:100, ICC/IF: 1:100-1:500, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 127-430 of human Cytokeratin 18.

Sequence

SHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

46 kDa

Predicted MW

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cell proliferation-inducing gene 46 protein, CK-18, CYK18, Cytokeratin-18, K18, Keratin, type I cytoskeletal 18, Keratin-18, KRT18

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Cytokeratin 18 Antibody (A90111)

Cytokeratin 18 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Cytokeratin 18 Antibody (A90111) at a dilution of 1:3000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-Cytokeratin 18 Antibody (A90111)

Cytokeratin 18 expression in human liver cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-Cytokeratin 18 Antibody (A90111) at a dilution of 1:100.

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Immunohistochemistry - Anti-Cytokeratin 18 Antibody (A90111)

Cytokeratin 18 expression in human small intestine tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-Cytokeratin 18 Antibody (A90111) at a dilution of 1:100.

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Immunofluorescence - Anti-Cytokeratin 18 Antibody (A90111)

Cytokeratin 18 expression in HepG2 cells analyzed by immunofluorescence. Staining was performed with Anti-Cytokeratin 18 Antibody (A90111) at a dilution of 1:300 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Anti-Cytokeratin 18 Antibody (A90111)

Rabbit polyclonal antibody to Cytokeratin 18 for WB, IHC-P, ICC/IF and ELISA.

Shipping Information

Specifications

Scientific Validation DataValidation Data (4)

Western Blot - Anti-Cytokeratin 18 Antibody (A90111)

Immunohistochemistry - Anti-Cytokeratin 18 Antibody (A90111)

Immunohistochemistry - Anti-Cytokeratin 18 Antibody (A90111)

Immunofluorescence - Anti-Cytokeratin 18 Antibody (A90111)

Alternative products to Anti-Cytokeratin 18 Antibody (A90111)

Proteins predicted to interact with Cytokeratin 18