Anti-Cytokeratin 19 Antibody [A53-B/A2] (Biotin) (A86358)

Name: Anti-Cytokeratin 19 Antibody [A53-B/A2] (Biotin)
Brand: Antibodies.com
SKU: A86358
Price: 125.0 GBP
Availability: InStock

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$210

Mouse monoclonal [A53-B/A2] antibody to Cytokeratin 19 (Biotin) for IHC-P, Flow Cytometry, IP, WB, ICC and ELISA.

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Alternative Formats and ConjugatesAlternative Formats

FITC Unconjugated

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Cytoplasm Marker Hepatocyte Marker

Specifications

Name

Anti-Cytokeratin 19 Antibody [A53-B/A2] (Biotin)

Description

Mouse monoclonal [A53-B/A2] antibody to Cytokeratin 19 (Biotin).

Specificity

This antibody recognizes the rod domain of Cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes. It is often co-expressed with Cytokeratin 7.

Applications

IHC-P, Flow Cytometry, IP, WB, ICC, ELISA

Dilutions

Flow Cytometry: 1-5 µg/ml; Intracellular staining, WB: 1-2 µg/ml.

Reactivity

Human

Immunogen

MCF-7 human breast adenocarcinoma cell line.

Host

Mouse

Clonality

Monoclonal

Clone ID

A53-B/A2

Isotype

IgG2a

Conjugate

Biotin

Purification

This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.

Concentration

1 mg/ml

Predicted MW

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store at 2-8°C. Do not freeze!

Synonyms

CK-19, Cytokeratin-19, K19, Keratin, type I cytoskeletal 19, Keratin-19, KRT19

Isotype Controls

Mouse IgG2a [MOPC-173] (Biotin) (A86310)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Anti-Cytokeratin 19 Antibody [A53-B/A2] (Biotin) (A86358)

Western blotting analysis of human cytokeratin 19 using Anti-Cytokeratin 19 Antibody [A53-B/A2] (Biotin) (A86358) on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 19 was detected at approximately 41 kDa.

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