Mouse monoclonal [A53-B/A2] antibody to Cytokeratin 19.
Specificity
This antibody recognizes the rod domain of Cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes. It is often co-expressed with Cytokeratin 7.
Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained with Anti-Cytokeratin 19 Antibody [A53-B/A2] (A86355), (concentration in sample 3 µg/ml, GAM APC).
Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using Anti-Cytokeratin 19 Antibody [A53-B/A2] (A86355), (diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange).
Western blotting analysis of human cytokeratin 19 using Anti-Cytokeratin 19 Antibody [A53-B/A2] (A86355) on lysates of HT-29 cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 19 monoclonal antibody A53-B/A2 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for cytokeratin 19 at approximately 40 kDa.
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