Recombinant humanized monoclonal antibody to EGFR.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA 1:5,000-1:10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1 (E239D, M241L)
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Immobilised Recombinant Human EGFR Protein (6xHis Tag) (A318318) at 2 µg/ml (100 µl/well) can bind Depatuxizumab Biosimilar - Anti-EGFR Antibody in a linear range of 0.13–16 ng/ml.
EGFR expresion in Expi293 cells compared to irrelevant protein as analyzed by flow cytometry. Staining was performed with 15µg/ml of Depatuxizumab Biosimilar - Anti-EGFR Antibody to obtain the blue histogram or the red histogram for the irrelevant protein.
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