Rabbit polyclonal antibody to DNA PKcs (Phospho-Thr2609)
Specificity
The antibody detects endogenous level of DNA PKcs only when phosphorylated at threonine 2609.
Applications
WB
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 2609 (V-E-T(p)-Q-A) derived from Human DNA-PK.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from K562 cells untreated (lane 1) or treated with hydroxyurea (lane 2) using DNA PKcs (Phospho-Thr2609) antibody #11167.
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