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Anti-DNP Antibody (CF®488A) (A344024)

CF®488A - Anti-DNP Antibody (CF®488A) (A344024) -Antibodies.com CF®488A - Anti-DNP Antibody (CF®488A) (A344024) -Antibodies.com CF®488A - Anti-DNP Antibody (CF®488A) (A344024) -Antibodies.com CF®488A - Anti-DNP Antibody (CF®488A) (A344024) -Antibodies.com CF®488A - Anti-DNP Antibody (CF®488A) (A344024) -Antibodies.com
$300
Product Datasheet

Rabbit polyclonal antibody to DNP (CF®488A) for ICC/IF, IHC, WB, Flow Cytometry and ELISA.

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Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-9 business days.

  • Specifications

Name
Anti-DNP Antibody (CF®488A)
Description
Rabbit polyclonal antibody to DNP (CF®488A).
Specificity
This antibody recognizes the dinitrophenyl (DNP) hapten and is suitable for detecting DNP-conjugated proteins and nucleic acids.
Applications
ICC/IF, IHC, WB, Flow Cytometry, ELISA
Dilutions
IF: 1–10 µg/mL, WB: 50–100 ng/mL, IHC: 1–10 µg/mL.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

CF®488A

Concentration
2 mg/mL
Product Form
Liquid
Reconstitution
CF® Dye and biotin conjugates: Add 0.5 ml dH2O for reconstitution. HRP or DNP conjugates: Add 1 ml dH2O for reconstitution.
Formulation
Supplied in Phosphate Buffered Saline containing 50% glycerol, 2 mg/ml BSA, and 0.05% Sodium Azide.
Storage
Shipped at +4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
General Notes

CF®488A is one of the most widely used green fluorescent dyes for biological imaging and flow cytometry. With excitation and emission maxima of 490/515 nm, it is compatible with standard 488 nm laser systems and serves as an excellent alternative to Alexa Fluor® 488 and FITC. CF®488A provides high signal intensity, outstanding photostability, and compatibility with advanced imaging techniques, making it ideal for demanding fluorescence applications requiring bright, stable labelling.

Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.

Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

CF®488A - Anti-DNP Antibody (CF®488A) (A344024) -Antibodies.comEnlarge Image

Anti-DNP Antibody (CF®488A) (A344024)

CF®488A exhibits substantially greater photostability than FITC. HeLa cells were stained with either CF®488A phalloidin or FITC phalloidin and imaged at time zero or after 5 seconds of continuous excitation using a FITC filter set. FITC fluorescence photobleaches almost immediately, whereas CF®488A retains strong signal intensity, demonstrating its high photostability.
CF®488A - Anti-DNP Antibody (CF®488A) (A344024) -Antibodies.comEnlarge Image

Anti-DNP Antibody (CF®488A) (A344024)

HeLa cells stained with rabbit anti-COX IV followed by CF®488A goat anti-rabbit to label mitochondria (green), and mouse anti-Golgin 97 followed by CF®555 goat anti-mouse to label the Golgi apparatus (red). Actin filaments are visualized with CF®405 phalloidin (blue), and nuclei are counterstained with RedDot™2 (magenta).
CF®488A - Anti-DNP Antibody (CF®488A) (A344024) -Antibodies.comEnlarge Image

Anti-DNP Antibody (CF®488A) (A344024)

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.
CF®488A - Anti-DNP Antibody (CF®488A) (A344024) -Antibodies.comEnlarge Image

Anti-DNP Antibody (CF®488A) (A344024)

Normalized absorption and emission spectra of CF®488A goat anti-mouse IgG in PBS.
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