Rabbit polyclonal antibody to EGFR (Phospho-Tyr1016)
Specificity
The antibody detects endogenous level of EGFR only when phosphorylated at Tyrosine 1016.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Tyrosine 1016 (D-E-Y(p)-L-I) derived from Human EGFR.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Hela cells using EGFR (Phospho-Tyr1016) Antibody #12013.The lane on the left is treated with the antigen-specific peptide.
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