Western Blot - Anti-EGFR (phospho Tyr1148) Antibody (A10875)
Western blot analysis of extracts of A-431 cells, using Anti-EGFR (phospho Tyr1148) Antibody (A10875) at 1:1,000 dilution. A431 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
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