Western Blot - Anti-EGFR (phospho Tyr869) Antibody (A306387)
Western blot analysis of extracts of NIH/3T3, using Anti-EGFR (phospho Tyr869) Antibody (A306387) at 1:1,000 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
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