Anti-EGFR (phospho Y1173) Antibody [EM-13] (A86778)

Western blotting analysis of EGFR (phospho-Tyr1173) using Anti-EGFR (phospho Y1173) Antibody [EM-13] (A86778) in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).

Separation of EGF stimulated A431 cell suspension stained with Anti-EGFR (phospho Y1173) Antibody [EM-13] (A86778), (concentration in sample 3 µg/ml, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining).

Immunoprecipitation of EGFR from EGF-treated A431 cells by monoclonal antibodies Anti-EGFR (phospho Y992) Antibody [EM-12] (A86777) (A), Anti-EGFR (phospho Y1173) Antibody [EM-13] (A86778), (B), a commercial Anti-EGFR polyclonal antibody (C) and Anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with A86777 or A86778, and goat anti-mouse-HRP.

Immunoprecipitation of EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies Anti-EGFR (phospho Y992) Antibody [EM-12] (A86777) (A), Anti-EGFR (phospho Y1173) Antibody [EM-13] (A86778), (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial Anti-EGFR polyclonal and Goat Anti-rabbit-HRP.