Anti-EpCAM Antibody [HEA-125] (A280587)

Anti-EpCAM Antibody [HEA-125]

Mouse monoclonal [HEA-125] antibody to EpCAM.

This antibody recognises CD326 also known as Adenocarcinoma-associated antigen, Epithelial glycoprotein 314 or Epithelial Cell Adhesion Molecule (Ep-CAM). CD326 is a 314 amino acid ~34 kDa single pass type I transmembrane glycoprotein nearing a single thyroglobulin type-1 domain (UniProt: P16422).CD326 is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells.CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells , and de novo expression being seen in dysplastic squamous epithelium. CD326 has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4.

IHC-P, IHC-Fr, IF

Flow Cytometry: Neat, IHC-Fr: 1:20, IHC-P: 1:20, Use 5ul of the suggested working dilution to label 10⁶ cells or 100µl cell suspension.

Human

HT29 carcinoma cell line.

Mouse

Monoclonal

HEA-125

IgG1

Lot Specific

Liquid

Supplied in Phosphate Buffered Saline with 0.2% BSA and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti human CD236 antibody, clone HEA-125 recognizes CD326 also known as Adenocarcinoma-associated antigen, Epithelial glycoprotein 314 or Epithelial Cell Adhesion Molecule (Ep-CAM). CD326 is a 314 amino acid ~34 kDa single pass type I transmembrane glycoprotein nearing a single thyroglobulin type-1 domain (UniProt: P16422).CD326 is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells.CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells (Balzar et al. 1999), and de novo expression being seen in dysplastic squamous epithelium (Winter et al. 2003). CD326 has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 (Proca et al. 2000) and Ber-EP4 (Patriarca et al. 2012).

Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, hEGP314, M1S2, M4S1, MIC18, TACSTD1, TROP1