Mouse monoclonal [ERB2/776] antibody to ErbB2 (CF®594).
Applications
Flow Cytometry, IF
Positive Control
SKBR-3 cells or breast cancer tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 0.5-1 µg/mL, IHC-P: 0.5-1 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Recombinant intracellular domain of human ErbB2 (HER2) protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
ERB2/776
Isotype
IgG1
Light Chains
kappa
Conjugate
CF®594
Concentration
0.1 mg/mL
Predicted MW
185 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.