Rabbit polyclonal antibody to ERK8 (Phospho-Thr175 / Tyr177)
Specificity
The antibody detects endogenous levels of ERK8 only when phosphorylated at threonine 175 and tyrosine 177.
Applications
WB
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of threonine 175 and tyrosine 177 (A-V-T(p)-E-Y(p)-V-A) derived from Human ERK8.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Jurkat cells, using ERK8 (Phospho-Thr175+Tyr177) antibody #12105. The lane on the right is treated with the synthesized peptide.
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