Anti-ERK8 (phospho Thr175+Tyr177) Antibody (A93516)

Name: Anti-ERK8 (phospho Thr175+Tyr177) Antibody
Brand: Antibodies.com
SKU: A93516
Price: 115.0 GBP
Availability: InStock

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$190

Rabbit polyclonal antibody to ERK8 (phospho Thr175+Tyr177) for WB and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-ERK8 (phospho Thr175+Tyr177) Antibody

Description

Rabbit polyclonal antibody to ERK8 (phospho Thr175+Tyr177).

Specificity

This antibody detects endogenous levels of ERK8 only when phosphorylated at Thr175 and Tyr177.

Applications

WB, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:40000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177 (amino acids 141-190).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

59kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ERK-7, ERK-8, ERK7, ERK8, Extracellular signal-regulated kinase 7, Extracellular signal-regulated kinase 8, MAP kinase 15, MAPK 15, Mitogen-activated protein kinase 15

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-ERK8 (phospho Thr175+Tyr177) Antibody (A93516)

Western blot analysis of lysates from Jurkat cells using Anti-ERK8 (phospho Thr175+Tyr177) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Western Blot - Anti-ERK8 (phospho Thr175+Tyr177) Antibody (A93516)

Western blot analysis of Jurkat cells using Anti-ERK8 (phospho Thr175+Tyr177) Antibody.

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Immunohistochemistry - Anti-ERK8 (phospho Thr175+Tyr177) Antibody (A93516)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-ERK8 (phospho Thr175+Tyr177) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.