This antibody recognizes CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
Applications
Functional Studies, Flow Cytometry
Dilutions
FUNC: In vitro induction of apoptosis, Flow Cytometry: 2-6 µg/ml.
Reactivity
Human
Immunogen
P815 cells transfected with human CD95.
Host
Mouse
Clonality
Monoclonal
Clone ID
EOS9.1
Isotype
IgM
Light Chains
kappa
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
46 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide (0.2 µm filter sterilized). Endotoxin level is less than 0.01 EU/µg of the protein.
Separation of Anti-Fas Antibody [EOS9.1] - Low endotoxin, Azide free (A86071) stimulated CEM cells (24 hours, 1 µg/ml, red-filled) from nonstimulated CEM cells (black-dashed) in flow cytometry analysis CEM cell suspension stained using ApoFlowEx kit.
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