Western Blot - Anti-FAS (phospho Y291) Antibody (A10861)
Western blot analysis of extracts of 293 and HeLa cells, using Anti-FAS (phospho Y291) Antibody (AP0007) at 1:1,000 dilution. 293 cells were treated by UV for 15-30 minutes. HeLa cells were treated by Doxorubicin (0.5uM) for 24 hours. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% BSA.
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