Anti-FGF2 Antibody [ARC51103] (A329369)

Name: Anti-FGF2 Antibody [ARC51103]
Brand: Antibodies.com
SKU: A329369
Price: 295.0 GBP
Availability: InStock

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$385

Rabbit monoclonal [ARC51103] antibody to FGF2 for WB, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-FGF2 Antibody [ARC51103]

Description

Rabbit monoclonal [ARC51103] antibody to FGF2.

Applications

WB, ICC/IF, ELISA

Positive Control

SKOV3, Mouse ovary, Mouse spleen, Rat spleen.

Dilutions

WB: 1:1,000-1:2,000, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to amino acids 100-200 of human FGF2.

Sequence

RAPERVGGRGRGRGTAAPRAAPAARGSRPGPAGTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51103

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

18 kDa / 22 kDa / 26 kDa

Predicted MW

31 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 0.05% BSA, 50% Glycerol, and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Basic fibroblast growth factor, bFGF, FGF-2, FGFB, Fibroblast growth factor 2, HBGF-2, Heparin-binding growth factor 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-FGF2 Antibody [ARC51103] (A329369)

FGF2 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-FGF2 Antibody [ARC51103] (A329369) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunofluorescence - Anti-FGF2 Antibody [ARC51103] (A329369)

FGF2 expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-FGF2 Antibody [ARC51103] (A329369) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunofluorescence - Anti-FGF2 Antibody [ARC51103] (A329369)

FGF2 expression in NIH/3T3 cells analyzed by immunofluorescence. Staining was performed with Anti-FGF2 Antibody [ARC51103] (A329369) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunofluorescence - Anti-FGF2 Antibody [ARC51103] (A329369)

FGF2 expression in PC-12 cells analyzed by immunofluorescence. Staining was performed with Anti-FGF2 Antibody [ARC51103] (A329369) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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