Anti-G-CSF Antibody [BVD13-3A5] (A279916)

Anti-G-CSF Antibody [BVD13-3A5]

Rat anti Human G-CSF

Rat anti Human G-CSF antibody, clone BVD13-3A5 recognizes human granulocyte colony stimulating factor (G-CSF), also known as Pluripoietin. In addition Filgrastim and Lenograstim are used to describe recombinant human G-CSF preparations. Human G-CSF is a 207 amino acid, ~20 kDa O-linked glycoprotein belonging to the IL-6 superfamily. There are potentially three isoforms formed by alternative splicing, Rat anti Human G-CSF antibody, clone BVD13-3A5 binds the canonical isoform-1, binding to other isoforms has not been assessed (UniProt: P09919).G-CSF is produced by activated macrophages, monocytes and neutrophils. In addition it can be produced by fibroblasts and endothelial cells (Zsebo et al. 1988). G-CSF stimulates production of cells belonging to the granulocytic lineage in the bone marrow and is an activating agent for mature neutrophilic granulocytes (Scapini et al. 2003) to produce other cytokines including CD257 (BAFF), operating through binding to the G-CSF receptor CD114.Rat anti Human G-CSF antibody, clone BVD13-3A5 has been successfully evaluated as a capture reagent in a highly sensitive sandwich ELISA assay with biotinylated Rat anti Human G-CSF, clone BVD11-37G10 (1012808) as a detection reagent and recombinant human G-CSF (PHP082B) as antigen.

WB, ELISA

Human

Recombinant human granulocyte-colony stimulating factor expressed in E. coil.

Rat

BVD13-3A5

IgG1

Affinity chromatography.

500 µg/ml

Liquid

Supplied in Borate Buffered Saline; without preservative.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

C17orf33, CSF3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin