Recombinant rabbit monoclonal [DM216] antibody to GFAP.
Applications
ELISA
Dilutions
ELISA: 1:5,000-10,000
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM216
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Anti-GFAP Antibody [DM216] - BSA and Azide free (A318494)
ELISA plate pre-coated by 1 µg/ml (100 µl/well) Recombinant Human GFAP Protein (6×His Tag) (A318077) can bind Anti-GFAP Antibody [DM216] - Azide free (A318494) in a linear range of 1-50 µg/ml.
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