This antibody reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system; it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Applications
WB, IHC-P, IHC-Fr, ICC
Dilutions
WB: 1-2 µg/ml.
Reactivity
Human, Porcine
Immunogen
Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Host
Mouse
Clonality
Monoclonal
Clone ID
GF-02
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Western blotting analysis of human GFAP using Anti-GFAP Antibody [GF-02] (A85479) on lysates of Neuro 2a and HEK 293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-GFAP monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GFAP at approximately 54 kDa.
Western blotting analysis (reducing conditions) of GFAP in porcine brain lysate. Lane 1: Immunostaining using Anti-GFAP Antibody [GF-02] (A85479)Lane 2: Immunostaining with Mouse IgM Isotype Control [PFR-03] (A86761).
Publishing research using Anti-GFAP Antibody [GF-02] (A85479)? Please let us know so that we can list the citation on this page.