This antibody reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system; it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Applications
WB, IHC-P, IHC-Fr, ICC
Dilutions
WB: 1-2 µg/ml.
Reactivity
Human, Porcine
Immunogen
Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Host
Mouse
Clonality
Monoclonal
Clone ID
GF-02
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Western Blot - Anti-GFAP Antibody [GF-02] (A85479)
Western Blotting analysis (reducing conditions) of GFAP in porcine brain lysate. Lane 1: Immunostaining with Anti-GFAP Antibody (A85479). Lane 2: Immunostaining with Isotype mouse IgM control (PFR-03).
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